Intracellular Delivery of Impermeable Molecules

Objective

Hydrosome Labs contracted MatTek Corporation, to show the ability of Hydrosome H2O to transport a cell impermeant molecule into living cells.

Design

Propidium Iodide, a popular red-florescent counterstain used in cell culture testing, is not permeable to living cells.  Both ultra pure water and Hydrosome H2O-processed water were introduced to 6 different types of cell cultures with propidium iodide added.

Results

The propidium iodide in the Hydrosome H2O samples were able to permeate live cells, while the Ultra Pure water with propidium iodide only permeated dead or dying cells.

Ultra Pure Water
Hydrosome H20

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