Objective
Hydrosome Labs contracted MatTek Corporation, to show the ability of Hydrosome H2O to transport a cell impermeant molecule into living cells.
Design
Propidium Iodide, a popular red-florescent counterstain used in cell culture testing, is not permeable to living cells. Both ultra pure water and Hydrosome H2O-processed water were introduced to 6 different types of cell cultures with propidium iodide added.
Results
The propidium iodide in the Hydrosome H2O samples were able to permeate live cells, while the Ultra Pure water with propidium iodide only permeated dead or dying cells.
- Hydrosome H2O + PI showed significant uptake of PI by live EpiIntestinal cells (SMI-100) at the 30 min and 120 min time points.
- Hydrosome H2O + PI treated skin epithelial tissues (EPI-200) showed uptake of PI by live skin epidermal cells at time 30 min.
- Hydrosome H2O + PI treated nasal tissues (NAS-100) showed PI uptake at 30 and 60 min.
Ultra Pure Water
Hydrosome H20
Find Out How We Can Advance Your Water-based Solution
